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KMID : 0366119740020030141
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Korean Journal of Applied Microbiology & Bioengineering
1974 Volume.2 No. 3 p.141 ~ p.147
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Studies on the Bacterial Production of L-Glutamate fromAcete
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Abstract
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The cultural conditions for L-glutamate production were investigated using Brevibacterium flavum nov. sp. D2209B, the most productive strain among 5 strains reported in preceeding paper.
Aterhperature of 30℃ and a medium volume of 30 ml per 500-flask were selected as standard culture conditions. And the following results were obtained.
1. When the concentration of acetate in the medium was below 30 g per litre, the maximum amount of L-glutamate was accumulated.
2. KH₂PO₄, MgSO₄, FeCI₃ and MnCl₂ were required for the L-glutamate poduction, but the concentration of those inorganic salts little effected.
3. Significant amount of L-glutamate was accumulated in the limited biotin concentration less than 0.3 ug per litre.
4. The addition of malic acid or succinic acid enhanced the accumulation.
5. The L-glutamate accumulation was related to the incubation time of seed; the amount of L-glutsmate accumulated was maximum by inoculating 16-20 hour incubated seed.
6. In the medium containing sufficient amount of biotin for growth, L-glutamate accumulation was .stimulated by the addition of penicillin at appropreate time during incubation.
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